Figure 3.

Change in the reversal potential of glycinergic PSPs. A, Glycinergic/GABAergic PSPs were evoked at 0.5 Hz in a cell exhibiting spontaneous bursts of simple spikes. Four segments from a 150-s-long V_m recording are shown in sequence. Complex spiking was induced after 20 s of control period by 75 pA injection (depol) for 30 s. The PSPs were depolarizing at a V_m of −79 mV during the pre-depolarization period. Just after the depolarization and complex spiking, PSPs were hyperpolarizing even at −81 mV but became depolarizing at −79 mV again over the next 100 s. PSPs were recorded in the presence of 100 μm APV and 10 μm DNQX. B, Evoked glycinergic/GABAergic PSPs shifting negatively after complex spiking. The bias currents during the pre-depolarization period for cells 1, 2, and 3 were −45, −60, and 0 pA, respectively, and the current was adjusted after depolarization to hold the V_m close to the pre-depolarization level.