Figure 7.
Activity-independent pHi and Egly change and simultaneous Cl and pH imaging. A, Examples showing the concurrent changes in Egly (left ordinate) and pHi (right ordinate) induced during and after 20 mm sodium propionate (Ai) and 10 mm TMA (Aii) perfusion for inducing intracellular acidification and alkalinization, respectively. SNARF images were obtained at 0.5 Hz while cells were held at −75 mV in v-clamp in TTX. B, Simultaneous two-photon imaging of intracellular pH and Cl− with SNARF and MQAE. Excitation wavelength, 750 nm. Bi, Two images of one area taken at the same time through the red emission channel (SNARF) and green channel (MQAE). MQAE was bulk-loaded into DCN slice, and SNARF was loaded from the recording pipette on the left of the cell at the lower left. Inhomogeneous staining with MQAE is typical. Bii, Simultaneous records of pHi (SNARF) and Cl−i (MQAE) obtained from two different cells. An 8 s complex spiking was evoked during the period of gray rectangle with the indicated amount of current. The peak of each signal is indicated with an arrow.