Fig. 6.

Overexpression of ADY4 decreases the rate of exchange of Spo21p and Spo74p in the absence of a prospore membrane. (A) Fluorescence recovery of Spo21p-GFP in sso1Δ cells overexpressing ADY4. Cells were analyzed during meiosis II. The green Spo21p-GFP plot represents the average of ten experiments. Fluorescence recovery of Spo21p-GFP in wild-type and sso1Δ cells from Fig. 1B is also shown for comparison. Error bars represent the s.d. at each time point. (B) Fluorescence recovery of Spo74p-GFP in sso1Δ cells overexpressing ADY4. The green Spo74p-GFP plot represents the average of ten experiments. Fluorescence recovery of Spo74p-GFP wild-type and sso1Δ cells from Fig. 1C are also shown for comparison. (C) Fluorescence recovery of Mpc54p-GFP in sso1Δ cells overexpressing ADY4. The green Mpc54p-GFP plot represents the average of eight experiments. Fluorescence recovery of Mpc54p-GFP wild-type and sso1Δ cells from Fig. 1D are also shown for comparison.