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. 2010 Jun 4;17(1):45. doi: 10.1186/1423-0127-17-45

Figure 2.

Figure 2

Effects of simvastatin on (A) thromboxane B2 formation, (B) phospholipase Cγ2 and (C and D) PKC substrate (p47) phosphorylation in activated platelets. Washed platelets were preincubated with simvastatin (30 and 50 μM) or 0.5% DMSO, followed by the addition of collagen (1 μg/ml) or PDBu (150 nM) to trigger platelet activation. Cells were collected, and subcellular extracts were analyzed for (A) thromboxane A2 formation, (B) phospholipase Cγ2 phosphorylation, and (C and D) phospho-PKC substrate (p-p47) as described in "Methods". Data are presented as the means ± S.E.M. (n = 4); *P < 0.05 and **P < 0.01, compared to the control group; #P < 0.05 and ##P < 0.01, compared to the collagen group.