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. 2010 Jun 15;10:67. doi: 10.1186/1471-213X-10-67

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Copyright ©2010 Tarnasky et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Ejaculates from XL169 chimaeric males contain spermatozoa that carry Odf2-β-geo. We analyzed whether or not high percentage chimaeric males produce spermatozoa and if they do whether or not these carry Odf2-β-geo DNA. Sperm ejaculates were indeed obtained from uterine horns of females mated to high percentage XL169 chimaeric mice and analyzed by genomic PCR using primers specific for Odf2-β-geo as described in Fig. 1 ("o"; lanes 2', 3', 4' and 5') and actin ("a"; lanes 2, 3, 4 and 5). Lane 1, marker DNA; lanes 2 and 3, C57Bl/6 sperm, which does not have Odf2-β-geo DNA; lanes 4 - 9, sperm from different chimaeric mice: note that they carry the Odf2-β-geo DNA.