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. 2010 May 20;10:216. doi: 10.1186/1471-2407-10-216

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Copyright ©2010 Miao et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Regulation of Nav1.8 mRNA and protein levels in bilateral DRG of tumor-bearing and sham rats. (A) Relative quantification (triplicate analysis of 4-6 rats for each time point) by real-time RT-PCR revealed a significant decrease in Nav1.8 mRNA in bilateral DRG of tumor-bearing rats compared with the sham group. (B) Western blots revealed a significant decrease in bilateral DRG of tumor-bearing rats compared with the sham group. Moreover, Nav1.8 protein expression was significantly downregulated on the ipsilateral side compared with the contralateral side of tumor-bearing rats. Data are expressed as mean ± SD. *P < 0.05 (one-way ANOVA) compared with sham rats.