Table 2.
List of genes involved in cell wall biogenesis
| Proteins | Genes in E. colia | Genes in P. aeruginosab | Functional Annotation |
|---|---|---|---|
| Penicilin Binding Proteins | |||
| PBP1a | ponA/mrcA | PA5045 | Membrane carboxypeptidase; Catalyzes the polymerization of the glycan subunit and crosslinking of muropeptides |
| PBP1b | ponB/mrcB | PA4700 | Membrane carboxypeptidase |
| PBP1c | pbpC | PA0378 | Transglycosylase |
| PBP2 | pbpA/mrdA | PA4003 | Chain-length regulation; Murein-elongation initiating enzyme |
| PBP3 | ftsI/pbpB | PA4418 | Transpeptidation of muropeptides exclusively for septal murein synthesis; septal murein-synthesizing enzyme; Important for cell dividion |
| PBP3a | None | PA2272 | Transpeptidase |
| PBP4 | dacB | PA3047 | D-alanyl-D-alanine endopepidase |
| PBP5 | dacA | PA3999? | D-alanyl-D-alaninecarboxypeptidase |
| PBP6 | dacC | D-alanyl-D-alanine carboxypeptidase; Stabilization of the murein at the stationary phase | |
| PBP6b | dacD | D-alanyl-D-alanine carboxypeptidase | |
| PBP7/8 | pbpG | PA0869 | D-alanyl-D-alanine carboxypeptidase; |
| Other proteins involved in bacterial cell shape | |||
| EnvA | envA/asmB/lpxC | PA4406 | UDP-3-O-acyl-N-acetylglucosamine deacetylase |
| RodA | rodA/mrdB | PA4002 | Rod shape-determining protein |
| RodX | rodX | None | |
| RodY/MreB/EnvB | rodY/mreB/envB | PA4481 | Rod shape-determining protein |
| MreC | mreC | PA4480 | Rod shape-determining protein |
| MreD | mreD | PA4479 | Rod shape-determining protein |
| Other proteins involved in bacterial cell wall metabolism | |||
| MpaA | mpaA | None | Gamma-D-glutamyl-meso-diaminopimelate hydrolase |
| MtgA | mtgA (mgt) | PA0378 | Glycan strand polymerization |
| Mpl | mpl | PA4020 | UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl-meso-diaminopimelate ligase |
| SltY | sltY | PA3020? | Lytic transglycosylase |
| MltA | mltA | PA1222 | Lytic transglycosylase |
| MltB | mltB |
PA4444/mltB1/sltB PA4001/sltB1/mltB2 |
Membrane-bound lytic murein transglycosylase B Lytic transglycosylase |
| MltC | mltC | PA3020? | Lytic transglycosylase |
| MltD | mltD | PA1812 | Lytic transglycosylase |
| MltE | mltE/emtA | PA3764 | Lytic transglycosylase |
| AmiA | amiA | PA5538 | N-acetylmuramoyl-L-alanine amidase; Cell separation |
| AmiB | amiB | PA4947 | Cell separationN-acetylmuramoyl-L-alanine amidase; Cell separation |
| AmiC | amiC | PA4947?/PA5538? | Cell separation |
| MepA | mepA | None | LD-endopeptidase |
| NagZ | nagZ | PA3005 | β-N-acetyl-D-glucosaminidase |
| LdcA | ldcA | PA5198 | LD-carboxypeptidase |
| EnvC | envC | PA5133 | Endopeptidase |
| FlgJ | flgJ | PA1085 | Muramidase (flagellum-specific) |
| Amp and Pox proteins | |||
| AmpC | ampC | PA4110 | AmpC beta-lactamase |
| AmpR | None | PA4109 | Transcriptional regulator AmpR |
| AmpD | ampD | PA4522 | 1,6-anhydro-N-acetylmuramyl-L-alanine amidase |
| ampDh2 | PA5485 | N-acetyl glucosamidase | |
| ampDh3 | PA0807 | N-acetyl glucosamidase | |
| AmpE | ampE | PA4521 | Hypothetical membrane protein |
| AmpG | ampG | PA4218 | Transport of degraded muropeptides: GlcNAc- anhMurNAc |
| AmpF | None | PA4219 | Hypothetical membrane protein |
| AmpP | None | PA4393 | Permease |
| AmpO | None | PA4219 | Hypothetical membrane protein |
| PoxA | None | PA5513 | Hypothetical hydrolase |
| PoxB | None | PA5514 | Class D beta-lactamase |
| Mur & Mra proteins | |||
| MurA | murA | PA4450 | UDP-N-acetylglucosamine enolpyruvyl transferase |
| MurB | murB | PA2977 | UDP-N-acetylpyruvoylglucosamine reductase |
| MurC | murC | PA4411 | UDP-N-acetylmuramate--alanine ligase |
| MurD | murD | PA4414 | UDP-N-acetylmuramoylalanine--D-glutamate ligase |
| MurE | murE | PA4417 | UDP-N-acetylmuramoylalanyl-D-glutamate-2, 6-diaminopimelate ligase |
| MurF | murF | PA4416 | UDP-N-acetylmuramoylalanyl-D-glutamyl-2, 6-diaminopimelate--D-alanyl-D-alanyl ligase |
| MurG | murG | PA4412 | UDP-N-acetylglucosamine--N-acetylmuramyl- (pentapeptide) pyrophosphoryl-undecaprenol N- acetylglucosamine transferase |
| MurI | murI | PA4662 | Glutamate racemase |
| MraY | mraY | PA4415 | Phospho-N-acetylmuramoyl-pentapeptide-transferase |
| MraW | mraW | PA4420 | Peptidoglycan biosynthetic process with methyl transferase activity |
| MraZ | mraZ | PA4421 | Role in cell-wall biosynthesis |
| MraR | mraR/ftsL | None | Cell division |