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. 2010 Jun 3;3:158. doi: 10.1186/1756-0500-3-158

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Copyright ©2010 Saier et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/2.0, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Alignment of the Consensus Sequence for the CDF Family (TMSs 5 and 6 and flanking regions) with the Consensus Sequence for the Orai Family (TMSs 3 and 4 and flanking regions). The consensus sequences were generated using the HMMER package (see Methods). The two consensus sequences were aligned using the GAP program (GCG package). This alignment shows 30% identity and 46% similarity with 3 gaps. TMSs were predicted with the TMHMM program http://www.cbs.dtu.dk/services/TMHMM. Each amino acid shown in the consensus sequence is the highest probability amino acid at that position according to the Hidden Markov Model.