Figure 2.
Summary of AG cell LY permeability with time. AG cells grown on permeable cell culture inserts were tested at various time points for their permeability to the hydrophilic macromolecule LY. The average LY permeability coefficient (Pcells expressed as ×10−6 cm s−1) for the weekly time points ±s.e. is shown against time. There was no statistical difference between LY permeability on day 7 (n = 34 inserts) and day 14 (n = 34 inserts). LY permeability decreased significantly from day 14 to day 21 (n = 30 inserts). After 21 days, LY permeability increased at both 28 (n = 18 inserts) and 35 days (n = 9 inserts) post-seeding. These results indicate that LY permeability and therefore barrier integrity was greatest approximately 21 days after seeding. *p < 0.07; **p < 0.02; ‡p < 0.01.