. Author manuscript; available in PMC: 2010 Jun 30.

Published in final edited form as: Bone. 2009 Feb 12;44(5):865–871. doi: 10.1016/j.bone.2009.01.393

Table 3.

Quantification of MMP-1, MMP-8 and MMP-13 protein levels from GCT stromal cell lysates and conditioned medium by a multiplex assay system

Conditioned media	MMP-1		MMP-8		MMP-13
Conditioned media	pg/mL	N^a	pg/mL	N^a	pg/mL	N^a
GCT-1	96.00±3.3	1.164	7.33±3.3	0.089	15.83±2.2	0.192
GCT-2	107.67±1.7	1.485	0.17±1.6	0.002	599.33±12.2	8.267
GCT-4	3.33±0.2	0.065	2.00±3.3	0.039	35.33±6.6	0.689
GCT-5	3495.33±105.7	50.841	0.00±2.8	0.000	301.83±41.3	4.390
GCT-7	13.00±0.9	0.067	0.00±0.8	0.000	32.67±2.6	0.168
GCT-9	567.5±18.4	3.266	1.33±1.7	0.008	142.17±6.6	0.818

Lysates	pg/mL	N^b	pg/mL	N^b	pg/mL	N^b

GCT-1	1374.17±165.9	60.0	53.0±6.0	2.3	901.83±124.5	39.4
GCT-2	15830.67±1598.0	1224.7	60.5±1.0	4.7	4185.67±231.6	323.8
GCT-3	13179.33±253.1	1529.7	64.33±3.9	7.5	27984.33±114.6	3248.2
GCT-4	140.5±50.0	11.1	49.0±4.1	3.9	2167.83±55.5	171.7
GCT-5	684.5±33.4	89.6	51.67±2.6	6.8	13887.83±449.7	1818.1
GCT-6	54.5±4.8	3.5	48.33±2.3	3.1	1685.83±21.1	107.2
GCT-7	254.83±13.4	9.4	45.5±1.3	1.7	1358.33±72.9	50.1

Mean data normalized (N) to pg per 1000 cells.

Mean data normalized to pg per 100 μg total protein.