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. 2010 Jun 30;5(6):e11391. doi: 10.1371/journal.pone.0011391

Figure 1. Cold exposure induces COX1 and COX2 expression in iWAT and iBAT.

Figure 1

Sv129 mice were warm-acclimated at 28–30°C for 6 days and then transferred to 4–6°C. Samples for cryosections, RNA and protein extractions were prepared from iBAT and iWAT after 2, 4 and 6 days at 4–6°C. A–B. Representative COX1 (A) and COX2 (B) immunoreactivity in iWAT from mice kept at 28–30°C for 6 days and after 6 days of cold exposure. C. Proteins were isolated from warm-acclimated mice (lane 1) and after 2, 4 and 6 days of cold exposure. COX1 and COX2 expression were determined by Western blotting. D. RNA was harvested from iBAT and iWAT from individual mice (n = 4 in each group) that were warm-acclimated or cold-exposed for 6 days. Expressions of genes were measured by RT-qPCR in duplicates and normalized to TBP (TATA box binding protein). The bars represent mean ± standard error. * indicates statistical difference (p<0.05) compared to expression in warm acclimated mice.