Figure 6. UCP1 expression is attenuated by an EP₄ receptor antagonist in Rb^−/− adipocytes and induced by the EP4 receptor agonist 16,16dmPGE₂ in vivo.

A. Expressions of EP₁, EP₂, EP₃, EP₄ and FP receptors were measured by RT-qPCR in iBAT and iWAT isolated from warm- and cold-acclimated mice, and in Rb^−/− adipocytes treated with vehicle or isoproterenol (100 nM) and 9-cis-retinoic acid (1 µM). B. Rb^−/− adipocytes were treated with vehicle or isoproterenol (100 nM) and 9-cis-retinoic acid (1 µM) by RT-qPCR in absence and presence of AL8810, AH6809, or AH23848, which are FP, EP₁/EP₂ and EP₄ receptor antagonists, respectively. Expressions of genes were measured by RT-qPCR. The bars represent mean ± standard error. Different letters indicate statistically significant differences (p<0.05). C. C57BL/6J mice were subcutaneously injected with vehicle or 16,16dmPGE₂ (50 µM/kg) every 12 h for 48 h. Expressions of genes were measured by RT-qPCR. The bars represent mean ± standard error (n = 5). * indicates statistical significant difference between vehicle and 16,16 dmPGE₂ treated mice (p<0.05).