Figure 7. Indomethacin prevents high-fat diet-induced UCP1 expression in iWAT but not iBAT in the obesity-resistant Sv129 mouse strain.

Mice were fed a very high-fat diet (VHF) with or without indomethacin supplementation (16 ppm) for 4 weeks at a temperature of 28–30°C. One group of mice was killed before the experiment started. A. Body weight gain and energy intake relative to body weight gain. B. Different adipose tissue depots were dissected and weighed. C and D. Representative paraffin-embedded representative sections from iWAT and iBAT were stained with hematoxylin and eosin. The scale bars represent 50 µM. E. Expressions of genes in iWAT and iBAT were measured by RT-qPCR. The bars represent mean ± standard error (n = 6). * indicates statistical significant difference (p<0.05) between different groups.