Figure 3.
Effect of dietary abscisic acid (ABA) supplementation on colon gene expression. C57BL/6J mice were fed ABA-supplemented (100 mg/kg) or control diets for 35 days and challenged with 2.5% dextran sodium sulfate (DSS) or water (no DSS) for 7 days. Expressions of peroxisome proliferator-activated receptor γ (PPAR γ) and PPAR γ-responsive genes CD36, fatty acid binding protein 4 (FABP-4) (A-C), adhesion molecules vascular adhesion molecule 1 (VCAM-1), muscosal vascular addressin cell adhesion molecule 1 (MAdCAM-1), E-selectin, intracellular adhesion molecule 1 (ICAM-1), and platelet/endothelial cell adhesion molecule 1 (PECAM-1) (D-H), and pro-inflammatory proteins interleukin-6 (IL-6), inducible nitric oxide synthase (iNOS), monocyte chemoattractant protein-1 (MCP-1), and matrix metalloproteinase 9 (MMP-9) (I-L) were assessed by real-time quantitative PCR. Data are represented as mean ± standard error. Points with an asterisk are significantly different from control, non-DSS treatment (P<0.05).