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. Author manuscript; available in PMC: 2011 Jan 1.
Published in final edited form as: Nat Methods. 2010 May 30;7(7):554–559. doi: 10.1038/nmeth.1463

Figure 2. Mutations in lin-35 and lin-15b enhance RNAi in neurons expressing sid-1.

Figure 2

(a) YFP expression in the nerve ring and ventral cord of worms with the indicated genotypes after feeding with bacteria making dsRNA for gfp or for mec-4 (compare with worms in Fig. 1). Both strains contain Punc-119yfp. Scale bars = 25 µm. (b) YFP fluorescence in the nerve ring of the indicated strains after feeding with bacteria making gfp dsRNA. The control strain is TU3310, expressing Punc-119yfp alone. Strains with Punc-119sid-1 were derived from TU3270 strain and have the mec-6(+) transgene. Results are presented as the mean percentage of fluorescence (± S.E.M.) measured in the same strain fed bacteria making mec-4 dsRNA (three experiments, each with 30 adult worms). (c) Anterior touch response in worms of the indicated strains fed bacteria making dsRNA for gfp (white) or mec-4 (gray). Values represent the mean ± S.E.M. of four experiments, each with 30 adults. The asterisk represents significance at p < 0.05.