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. Author manuscript; available in PMC: 2011 Jul 1.
Published in final edited form as: J Cell Biochem. 2010 Jul 1;110(4):846–856. doi: 10.1002/jcb.22593

Fig. 3. Differential hypophosphorylation of activating ad inactivating tyrosines of Lyn kinase by PTPROt.

Fig. 3

Lyn-HA was immunoprecipitated from whole cell extracts of H293T cells co-expressing HA-tagged Lyn and Flag-tagged PTPROt. The extracts along with the immunoprecipitated complex were separated on SDS-PAGE (in duplicate) followed by immunoblotting either with anti-pY(416)Src (A) or anti-pY(507)Lyn (B). The same blot was reprobed with anti-HA to measure total Lyn. Quantification of phosphorylation of the tyrosine residues of Lyn normalized to Lyn-HA is provided in the respective panels. The experiment was repeated three times with similar observation. One representative data was quantified for illustration.