Figure 3.

SB and VPA treatment results in inhibition of CMP differentiation. CD34⁺ cells were cultured in the presence of G-CSF to induce neutrophil differentiation. Cells were cultured in either the absence or presence of TSA (5–25 nM), SB (100–500 μM) or VPA 100–500 μM). At days 3 and 7, a progenitor staining was performed. CD34⁺CD38⁺ cells were characterized by FACS based on CD123 and CD45RA expression as CMP (CD123⁺/CD45RA⁻), GMP (CD123⁺/CD45RA⁻) or MEP (CD123⁻/CD45RA⁻). Data are expressed as the percentage of CMP (A–B), percentage of GMP (C–D) and percentage of MEP (E–F) at day 3 (A, C, E) and day 7 (B, D, F). CMP, GMP and MEP represent a percentage of total CD34⁺ cells. Error bars represent SEM (between experiments, n=3) * P<0.05, ** P<0.01.