Figure 4.

HDAC inhibition affects CD34⁺ progenitor differentiation and lineage commitment. CD34⁺ cells were cultured in the presence of G-CSF and EPO for 11 days to induce CFU-GM and CFU-E. Cells were cultured in either the absence or presence of TSA (5–25 nM), SB (100–500 μM) or VPA (100–500 μM). Each plate was scored for granulocyte/macrophage colony-forming unit and erythroid burst/colony forming unit growth. Data are expressed as the number of total colonies (A) (n=5), photos were taken to demonstrate GM-colony size (B) and data are expressed as the percentage of granulocyte/macrophage colonies (C) and erythroid colonies (D) (n=4). Error bars represent SEM (between experiments) *P<0.05, **P<0.01.