Figure 4.

SAHA reduces cell migration and synergizes with fludarabine in a mesenchymal stromal cell/CLL co-culture model. (A) CLL cells were cultured in the presence or absence of SAHA for 24 h before plating onto 5-μm Transwell microporous membranes for the migration assay. Results are expressed as a mean (± SEM) of the migration index. Cell viability normalized to untreated (B) and percentage of apoptotic cells (C) were evaluated after 48 h of incubation with SAHA (20 μM) in the presence or absence of a stromal layer. Cell viability normalized to untreated (D) and percentage of apoptotic cells (E) were evaluated on mononuclear cells pre-incubated for 4 h with SAHA (1 μM) and then incubated with SAHA and fludarabine for 48 h on a stromal layer.