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An associated 5′ ftz intron provides a substantial increase in reporter gene expression, with or without internal shmiRs, in Drosophila cells. Equal DNA concentrations of Renilla luciferase (RLTK), 5′ ftz RLTK, 5′ ftz RLTK with a single intronic mCherry shmiR, or 5′ ftz RLTK with tandem intronic mCherry shmiRs, along with firefly luciferase, were transfected into S2 cells. All constructs were expressed with the actin5C promoter.
