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HAfp1–23 backbone dynamics extracted from 15N relaxation rates at a magnetic field strength of 14.1 Tesla, pH 7.4 (black) and 4.0 (red). (A) Longitudinal 15N relaxation rates, R1; (B) Transverse 15N relaxation rates, R2; (C) 15N-{1H} NOE; (D) Model-free generalized order parameters, S2, derived from these data for a librationally corrected 1.04 Å bond length and an axially symmetric 15N Δσ value of -173 ppm applicable for α-helices (57), using an isotropic diffusion model.
