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X-band EPR spectra of GcpE ± reactants/products. (A) EPR of E. coli GcpE reduced with 20 equivalents Na2S2O4. (B) EPR of E. coli GcpE + MEcPP, incubated for 1 min. (C) EPR of E. coli GcpE + HMBPP epoxide, incubated for 2 min. (D) EPR of E. coli GcpE + MEcPP, incubated for 45 min. (E) EPR of E. coli GcpE + HMBPP, incubated for 45 min. Frequency = 9.05 GHz, microwave power = 1 mW for A, 0.05 mW for B–E, temperature = 15 K.
