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Comparative analysis of in vitro transcriptional assay of reconstituted holoenzyme with HrdB115 protein or its mutant HrdBA56 protein on aveRp promoter. In all lanes, the holoenzyme was reconstituted by 0.25 pmol E. coli RNA polymerase core enzyme (EPICENTRE) with the indicated amounts of σhrdB. The ratio of sigma factor to the core RNA polymerase is indicated.
