Turnover in the FliM-YPet complex. (A, B) Pre- and post-focused-laser bleach images, showing immobilized (Upper) and tethered (Lower) cell images for: (A) FliM-YPet and (B) FliM-YPet/ΔCheY. Typical examples of spots analyzed for FRAP (Red Arrow) and FLIP (Blue Arrow) are shown. (C–F) Mean FRAP (Red) and FLIP (Blue) traces (SEM error bounds shown as dotted lines) for: (C) FliM-YPet, (D) FliM-YPet/ΔCheY, (E) FliM-YPet/ΔCheY/CheYD13K/Y106W, and (F) FliM-YPet/ΔCheY/CheYD57A strains. Each trace is based on 7–11 spots.