FIG. 2.
Detection of HIV-1 in microcultures after cultivation of PHA-PBMC from infected patients with primary cells and cell lines. A total of 5 × 105 PHA-stimulated, CD8-depleted PBMCs from HIV-1-infected patients were cocultured with 5 × 105 cells from the cell lines or 2 × 106 PHA-stimulated, CD8-depleted PBMCs from a seronegative donor for 7 days. During this time supernatant p24 was measured by ELISA. Results are expressed as optical density obtained in ELISA. For each patient, duplicate experimental wells were assayed in triplicate by p24 ELISA. The percents in the boxes indicate the percentage of patient cultures in which HIV infection could be detected, as defined by p24 levels 2 SD greater than plate mean background. Open triangles represent the mean value of all positive cultures and filled circles represent the median. The top of the thick bar is the 75% percentile and the bottom is the 25% percentile. The lines demonstrate the full range of values.