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. Author manuscript; available in PMC: 2011 Jun 22.
Published in final edited form as: Biochemistry. 2010 Jun 22;49(24):5048–5056. doi: 10.1021/bi1005046

Figure 2.

Figure 2

Metal affinity of HDAC8. The activity of HDAC8 was assayed in the presence of increasing concentrations of free Zn2+ (diamonds) or Fe2+ (circles) using a metal buffer, as described in Materials and Methods. The relative initial velocity (vobs/vmax) is plotted to simplify the graph. Under these conditions the activity of Fe2+-HDAC8 is 2.8-fold higher than that of Zn2+-HDAC8 (27). The metal dissociation constant was determined from fitting a single binding isotherm (Eq. 1) to these data.