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. 2010 Jul 1;24(13):1364–1376. doi: 10.1101/gad.1917310

Figure 1.

Figure 1.

Loss of pRB induces CIN. (A,B) FISH analysis with probes for chromosomes 6 and 8 revealed a high degree of aneuploidy in populations of pRB-depleted RPE-1 (sh-Rb) cells that persisted following several weeks of chronic depletion. Both control and sh-Rb populations exhibited a modal copy number of two for both chromosomes 6 and 8. (C) Analysis of individual segregation events revealed high rates of chromosome segregation errors in pRB-depleted cells (0.87% and 0.64% segregation error/division for chromosomes 6 and 8, respectively). (D) Following chronic depletion of pRB, aneuploid cells continued to proliferate (prometaphase cell) (top panel) and to missegregate chromosomes (late anaphase/telophase cell) (bottom panel). Chromosome 6 is shown in green, and chromosome 8 is shown in red throughout. Bar, 5 μm.