Table 1.
Anaerobic and aerobic maximal specific growth rate determination
The anaerobic-specific growth rates were determined in fully anaerobic controlled batch fermentations. The aerobic-specific growth rates were measured in shake flasks. The right-hand column displays the fitness reduction in aerobic competitive chemostats of the five mutants that showed a significant disadvantage in anaerobic competitive chemostats. The μmax data of the mutants were compared to the reference strain (ygr059wΔ) data by means of a t test. The significance of the difference is indicated by the P value (P>0.05). Data are presented as the mean±sd of results from two independent cultures for each strain.
| Deletion mutant | Anaerobic batch | Aerobic batch (shake flask) | |||
|---|---|---|---|---|---|
| μmax (h−1) | t test P value | μmax (h−1) | t test P value | Fitness reduction (%) | |
| plb2Δ | 0.31±0.01 | 7.05×10−2 | 0.39±0.00 | 3.99×10−1 | 7.0±3.7 |
| ylr413wΔ | 0.25±0.08 | 3.83×10−1 | 0.38±0.02 | 6.07×10−1 | 11.6±3.3 |
| izh2Δ | 0.32±0.00 | 1.31×10−2 | 0.38±0.01 | 4.87×10−1 | 15.8±7.4 |
| eug1Δ | 0.29±0.01 | 3.42×10−2 | 0.37±0.02 | 6.38×10−1 | 8.6±0.1 |
| yor012wΔ | 0.29±0.00 | 4.05×10−3 | 0.34±0.00 | 3.15×10−3 | 14.8±5.6 |
| ygr059wΔ | 0.34±0.00 | − | 0.40±0.02 | − | − |
| CEN.PK 113-7D | 0.34±0.01 | − | 0.39±0.00 | − | − |