Table 1.
Oligonucleotides used in this study
| Name | Sequence | Application* |
|---|---|---|
| CA_HMG_F+START | ATGTTGAGATCATTTGTAACATC (23-mer) | 1, 3 |
| CA_HMG_R | AAAGTCATCCTCCACTTTGTAG (22-mer) | 1, 4 |
| CA_HMG_R+STOP | TCAAAAGTCATCCTCCACTTTG (22-mer) | 1 |
| 5BamORF | ATGGATCCATGTTGAGATCATTTGTAACA (29-mer) | 1, 2 |
| REsp3ORF | ATCGTCTCTACATAAAGTCATCCTCCACTTTGTA (34-mer) | 1 |
| 5GFPEsp3 | ATCGTCTCAATGTCTAAAGGTGAAGAATTA (30-mer) | 1, 3 |
| 3PstGFP | ATCTGCAGTTATTTGTACAATTCATCCAT (29-mer) | 1 |
| 3PstORF | ATCTGCAGGTCAGTAATTCACTTTTCCGG (29-mer) | 1, 3, 4 |
| R3OUT | ATGAGCTCTATTTCTCGAATCAGTTGATT (29-mer) | 1, 2, 3 |
| D3orf | ATTGCGGCCGCCTCAATCAACTAAAAGAGGTC (32-mer) | 1 |
| R5orf | ATACTCGAGAGATGAACAACTAAAATTGAC (30-mer) | 1 |
| D5OUT | ATGGGCCCACTAATATTTTACCTGCAGGG (29-mer) | 1 |
| pMET | ATGAAAAGACAAGAGAGC (18-mer) | 4 |
| GFP | AGATGGTGATGTTAATGG (18-mer) | 4 |
| pGEX5 | GGCAAGCCACGTTTGGTG (18-mer) | 4 |
| pGEX3 | GGAGCTGCATGTGTCAGAGG (20-mer) | 4 |
| 5ORFterm | ATCTGCAGAGAACTCAATCAACTAAAA (27-mer) | 3 |
| D3OUT | ATTGCGGCCGCCGATGGATGGATGACGAGAAC (32-mer) | 2 |
| IMH3OUT | AGAGGTATGGGTTCCATCGAT (21-mer) | 3 |
| 3PstKO | ATCTGCAGTGCCTTCTCTTCCTCGGGGAA (29-mer) | 2 |
| CaAbf2Rev | TCAATAGTCAAATAAAGTAGC (21-mer) | 3 |
| CaUra3in3 | TAATTGGCCAGTCTTTTTCAAATAAGC (27-mer) | 2 |
| CamtHMG1in5 | GTGGTACCATCACCGACTTCA (21-mer) | 3 |
| CamtHMG1in3 | ATCCGCAGCAACCGTCAATT (20-mer) | 3 |
| MPAout3 | AGATCCACTAGTTCTAGAGCGGCC (24-mer) | 3 |
| MPAout5 | CTAGAAAGTATAGGAACTTCCTCGAG (26-mer) | 3 |
| D5R5in | ATATTCTCCATTATACTTTGTATTGG (26-mer) | 2 |
| D3R3in | AACTATGGTCAAAAGGGTCTAAAAA (25-mer) | 2 |
| CanEco31 | ACGTGGTCTCATGGAATTAACACCCACACAG (31-mer) | 2 |
| Can3CBam | GCGGATCCTAAATTAGTCGACTAGT (25-mer) | 2 |
| 5RTcaAct1 | TGTCTTTGTACTCTTCTGGTAGAAC (25-mer) | 5 |
| 3RtcaAct1 | CTAGTAGTGAAACTGTAACCACGTT (25-mer) | 5 |
| 5RTcaGCF1 | CAAAAGCTATTTTACTCCTAAACCA (25-mer) | 5 |
| 3RTcaGCF1 | ATCTTCTTTACTGATTTGGTATTGC (25-mer) | 5 |
| 5RTcaNAD2 | TTCAAAATGGGATTAGCACCTT (22-mer) | 5 |
| 3RTcaNAD2 | TGTATATGCTGCCACGGCTA (20-mer) | 5 |
*1, PCR amplification for cloning; 2, PCR amplification to prepare probe for Southern blot hybridization; 3, PCR verification; 4, sequencing; 5, qRT-PCR.