Table I.
Thermodynamic Parameters for Interaction of Peptide with SecB
Protein species | Kd (μM) | ΔG (kcal/mol) | ΔH (kcal/mol) | TΔS (kcal/mol) |
---|---|---|---|---|
SecB | 32 ± 1 | −5.78 | 2.79 ± 0.07 | 8.57 |
SecB145 | 35 ± 3 | −5.73 | 2.06 ± 0.08 | 7.79 |
SecB144 | 52 ± 2 | −5.50 | 2.17 ± 0.07 | 7.67 |
SecB143 | 85 ± 7 | −5.23 | 1.99 ± 0.18 | 7.22 |
SecB142 | 224 ± 23 | −4.69 | 2.51 ± 0.20 | 7.20 |
The relationships used to obtain Kd and TΔS are: Kd = 1/Ka, ΔG = −RTlnKa, and ΔG = ΔH − TΔS.