Figure 2.
One-step growth curve: VSV23 (diamond), VSVST (circle), VSVXN2 (square), and VSVwt (triangle) were used to infect L929 cells at an MOI = 1 in triplicate. Media samples were removed at 1.5, 3, 6, 12, and 24 hours and stored at −80 °C. Virally infected supernatants were serially diluted and transferred to fresh L929 cells for plaques assay. Growth kinetics for all viruses are similar at all time points. Data presented are geometric means ± standard deviation and are representative of three replicate experiments.
Abbreviations: PI, post infection, MOI, multiplicity of infection; VSV, vesicular stomatitis virus.