Table 2.
Contents of PHB and PHBV in haloarchaeal species, determined by proton NMR from cell extracts
| Origin of material | ||||||
|---|---|---|---|---|---|---|
| Organism | Freeze-dried cells | Fresh cells, aqueous extract | Fresh cells, hypochlorite extract | |||
| PHB | PHBV | PHB | PHBV | PHB | PHBV | |
| w/w% of cell dry weight | w/w% of cell dry weight | w/w% of dried extract | w/w% of dried extract | w/w% of dried extract | w/w% of dried extract | |
| Hfx. mediterranei DSM 1411T | 0.19 | 0.03 | 69.6 ± 0.43a | 8.1 ± 0.3a | ND | ND |
| 0.20 | 0.05 | |||||
| Hfx. volcanii DSM 3757T | ND | ND | Not detectable | Not detectable | ND | ND |
| Hcc. dombrowskii DSM 14522T | 0.15 | 0.01 | 0.4 ± 0a | 0.07 ± 0.05a | 0.1 | Not detectable |
| Hcc. salifodinae DSM 8989T | 0.05 | 0.01 | ND | ND | ND | ND |
| Hcc. saccharolyticus DSM 5350T | ND | ND | 1.2 | Not detectable | 0.05 | Not detectable |
| Har. hispanica DSM 4426T | 0.09 | 0.04 | ND | ND | ND | ND |
| Hbt. salinarum R1 | Not detectable | Not detectable | ND | ND | ND | ND |
| Hbt. salinarum NRC-1 ATCC 700922 | ND | ND | Not detectable | Not detectable | Not detectable | Not detectable |
| Hbt. noricense DSM 9758T | 0.08 | 0.03 | ND | ND | ND | ND |
| Hqr. walsbyi DSM 16790 | ND | ND | 0.1 | Not detectable | ND | ND |
| Nbt. gregoryi NCMB 2189 T | 0.1 | 0.03 | 0.1 | Not detectable | 0.4 | Not detectable |
| 0.44 | 0.18 | |||||
| Ncc. occultus DSM 3396 T | ND | ND | ND | ND | 3.1 | Not detectable |
Freeze-dried cells were treated with CDCl3 and sonicated prior to 1H NMR analysis (see Methods); cell dry weights ranged from 33 to 170 mg. Water and hypochlorite extracts were prepared from fresh cells (see Methods for details) and ranged from 2 to 10 mg
ND not done
aMean value ± standard deviation from three experiments each