Fig. 3.
Confocal overlay (MAP2 and NF200) images of neurons at DIV 10. MAP-2 staining is seen in green and NF200 staining in red. Panel a represents neuronal morphology in control group at DIV 10. Panel b shows neuronal morphology in RU486-treated neurons at DIV 10. Panels C and D show neuronal morphology in LPS-treated group at DIV 10. The control and RU486 cells demonstrated numerous processes present with discrete cellular morphology. The LPS-exposed neurons demonstrated decreased growth, abnormal morphology and decreased MAP2 staining. In the addition, the MAP2 staining in LPS treated neurons has a discontinuous dotted pattern. Magnification 400×.