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. Author manuscript; available in PMC: 2010 Jul 1.
Published in final edited form as: J Biol Chem. 2007 Apr 12;282(22):16105–16116. doi: 10.1074/jbc.M611115200

FIGURE 5. FADDDN blocks cell death in IKKα−/− but not IKKβ−/− MEFs.

FIGURE 5

A, WT, IKKα−/−, or IKKβ−/− MEFs were either untreated (shaded) or treated overnight with TNF (10 ng/ml; unshaded) in the absence (Control) or presence of zVADfmk (25 μM). FADD DN-transduced IKKα−/− and IKKβ−/− MEFs were also either untreated or treated overnight with TNF. Cells were harvested, incubated in PBS containing PI, and then analyzed by FACS. Membrane-permeable cells that took up PI were determined in region M1, and the percentage of PI-positive cells is shown in B.