FIGURE 8. Effects of TNF and zVADfmk on Mn-SOD and catalase levels in WT and IKKβ^−/− MEFs.

WT and IKKβ^−/− MEFs were either untreated or incubated overnight with TNF (10 ng/ml) in the absence or presence of zVADfmk (25 μM) as shown. Cell lysates were separated by SDS-PAGE (10%), and the resulting immunoblots were probed using either anti-Mn-SOD (A) or anti-catalase (B). Anti-tubulin was used to ensure equal protein loading.