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A, Confocal image of saponin-permeabilized rat ventricular myocyte with 20 nM F-FKBP12.6; B, plot profile of striated sarcomeric pattern and sinusoidal fit; C. longitudinal line scan image from myocyte exposed to 100 nM F-FKBP12.6; D, simultaneous Ca spark measurement using rhod-2 as Ca indicator; E, Merged C-D images showing Ca sparks originate from FKBP binding sites.
