Figure 3. FKBP12.6-RyR2 association and resting RyR2 activity.
A, Time course of FKBP12.6 (100 nM) wash-in and influence of cAMP and rapamycin; B, Normalized FKBP12.6 and cAMP effects on CaSpF, FKBP12.6-RyR2 binding (n=25,17) & SR Ca load (assessed by 10mM caffeine, n=6). Basal control CaSpF is 8.7± 0.6 sparks (s-1 100μm-1). C, Normalized graph of rapamycin effects on CaSpF, FKBP12.6-RyR2 binding, and SR load (n=45,10,7). D-F, FKBP12.6, rapamycin & cAMP effects on Ca spark amplitude, width (FWHM) and duration (FDHM).