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. Author manuscript; available in PMC: 2011 Jun 11.
Published in final edited form as: Circ Res. 2010 Apr 29;106(11):1743–1752. doi: 10.1161/CIRCRESAHA.110.219816

Figure 4. Steady-state Kd and Bmax of F-FKBP in myocytes.

Figure 4

A, Confocal myocyte image with 20 nM bath [FKBP12.6]; B, Same as A but myocyte was pretreated with 20 μM rapamycin; C, Dependence of bath F-FKBP12.6 fluorescence on [F-FKBP12.6]. D, Background-corrected (bath & auto-fluorescence) fluorescence intensity from Z-line, M-line and average, plotted vs. bath [FKBP12.6]. E, Calibrated F-FKBP12.6 binding using known bath concentration and fluorescence (fit with one-site isotherm; n=4 rats). F, Influence of cAMP/OA pretreatment on apparent Bmax and Kd (n=4 rats).