Fig. 4.

Iterative screening of OOXX samples using in vivo antinociception: summed antinociception produced by 1802 series samples (5 mg/kg, i.p.) measured in the mouse 55°C warm water tail-withdrawal test across a 24-h testing period. Samples were defined in the first position (inset key) and second position (see Table II) identified on the x-axis. The combined time to withdraw tails (s; y-axis) was calculated by taking the sum of the average tail withdrawal latencies from each time point. Functionalities of key samples are described in simplified form for convenience; see Table II for complete descriptions. Note the inclusion of morphine (10 mg/kg, i.p., far right bar) as a positive control. Data represent average (±SEM) summed tail-withdrawal latencies calculated by taking the sum of the average tail withdrawal latencies for each animal from each time point over a 24-h period. Samples were administered at a dose of 5 mg/kg, i.p. Bars, 8 mice each. *Significantly greater than morphine effect, p < 0.05, Student’s t test