Fig. 8.

TPI 1818-101 and 1818-109 do not induce morphine-like conditioned place preference. a Schematic of testing protocol. b Summary graph of place preference results. Initial preference for either the left or right compartment of the CPP system was determined on day 1 of testing for each group of mice (solid bars) and plotted as the difference in time spent on the eventual drug-paired side (s). On days 2 and 3, individual mice were place conditioned in the initially preferred chamber with vehicle (0.9% saline) followed 6 h later by place conditioning in the initially non-preferred chamber side with morphine (white bars), vehicle (10% DMSO in 0.9% saline, gray bars), TPI 1818-101 (green bars), or 1818-109 (red bars). On the fourth day of testing, mice were again tested to determine final place preference (striped bars). Final conditioned place preference did not significantly differ from the initial preference for mice treated with vehicle, TPI 1818-101, or 1818-109, but was significant for morphine. Each bar represents 8–16 mice. *Significant difference from the initial preference (p < 0.05). ^†Significant difference from final preference of morphine treated mice (p < 0.05). ^‡Significant difference from saline place-conditioned animals (p < 0.05)