Table III.
Radiolabeled ligands | K i (nM) ± SEM | ||
---|---|---|---|
[3H]DAMGO (MOR) | [3H]DPDPE (DOR) | [3H]U69,593 (KOR) | |
TPI 1818-101 | 2,790 ± 741 | 1,930 ± 474 | 3,910 ± 1,640 |
TPI 1818-109 | 9,470 ± 1,210 | 2,210 ± 932 | 7,750 ± 3,830 |
Murine brain membrane protein was incubated with six different concentrations of each TPI compound in the presence of 0.87 nM [3H]DAMGO, 2 nM [3H]DPDPE, or 2 nM [3H]U69,593 in 50 mM Tris–HCl, pH 7.5, at 25°C as described in “MATERIALS AND METHODS.” Data are listed as the mean K i values ± SEM from at least three experiments