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Effect of leptin on the expression of Ob-Ra and Ob-Rb mRNA in differentiated THP-1 cells. Cells were incubated with increasing concentrations of leptin (0.01-10 µg/mL) in the absence (A) or presence (B) of Prevotella intermedia lipopolysaccharide (LPS) (0.01 µg/mL). See MATERIALS AND METHODS for further details. The polymerase chain reaction bands on a gel photograph in one of two separate experiments yielding similar results are shown.
