Figure 3.

Increasing extracellular Ca²⁺, [Ca²⁺]_o, potentiated PAF-induced increases in endothelial [Ca²⁺]_i, NO production, and microvessel permeability. (A) Endothelial [Ca²⁺]_i measurements from two of the experiments showing that increasing [Ca²⁺]_o potentiates PAF-induced increases in endothelial [Ca²⁺]_i. (B) High [Ca²⁺]_o further increased PAF-induced NO production, as indicated by DAF-2 cumulative FI curves (open triangle vs. filled circle) and NO production rate (dashed line vs. solid line). (D) Lp measurements from two of the experiments showing a potentiated Lp increase relative to that measured with normal [Ca²⁺]_o, which correlated with high magnitude increases in endothelial [Ca²⁺]_i and NO production.