Table 2.
CBC-LAMP assay sensitivity from pure DNA
| Detection method | Purified Xanthomonas citri subsp. citri DNA | ||||||||
|---|---|---|---|---|---|---|---|---|---|
| 100 ng | 10 ng | 1 ng | 100 pg | 10 pg | 1 pg | 100 fg | 10 fg | 1 fg | |
| Gel | + | + | + | + | + | + | + | + | - |
| LFD | + | + | + | + | + | + | + | + | - |
| SYBRGreen | + | + | + | + | + | + | Nc | Nc | - |
For each dilution the CBC-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick.+: Positive reaction.-: Negative reaction. Nc: The colour developed in the test tube was not clearly distinguishable between a positive or negative reaction.