Table 3.
CBC-LAMP assay sensitivity from cultured cells and infected tissue
| Strain | Specimen source |
Detection method | CFU per reaction (10-fold dilutions) |
|||
|---|---|---|---|---|---|---|
| X. citri pv. citri | Pure culture | 395.3 | 37.6 | 5.2 | 0.7 | |
| Gel | + | + | + | - | ||
| LFD | + | + | + | - | ||
| SYBRGreen | + | + | + | - | ||
| X. citri pv. citri | Infected tissue | 248.4 | 18.7 | 3.3 | 0.2 | |
| Gel | + | + | - | - | ||
| LFD | + | + | - | - | ||
| SYBRGreen | + | + | - | - | ||
For each dilution the CBC-LAMP reaction was performed in triplicate. Gel: gel electrophoresis. LFD: lateral flow dipstick. +: Positive reaction.-: Negative reaction.