Table 4.
Strains of Citrus pathogenic Xanthomonas used to evaluate the CBC-LAMP assay
| Species | Strain (s) | Origin | CBC type | Detection Method | ||||
|---|---|---|---|---|---|---|---|---|
| Host | Place | Country | Gel | LFD | S G | |||
| Xanthomonas citri subsp. citri | XC1CE | Tangerine | Concordia, Entre Rios | Argentina | A | + | + | + |
| XC2COE | Orange | Colon, Entre Rios | Argentina | A | + | + | + | |
| XC3AM-1, XC3AM-2 | Lemon | Apostoles, Misiones | Argentina | A | + | + | + | |
| XC4PM | Grapefruit | Posadas, Misiones | Argentina | A | + | + | + | |
| XC5LF-1, XC5LF-2 | Grapefruit | Las Lomitas | Argentina | A | + | + | + | |
| XC7ETS-1, XC7ETS-2 | Orange | El Tabacal, Salta | Argentina | A | + | + | + | |
| XC8SPB-1, XC8SPB-2 | Orange | San Pedro, Buenos Aires | Argentina | A | + | + | + | |
| XC9CAT -1, XC9CAT-2 | Orange | Catamarca | Argentina | A | + | + | + | |
| XC10BVC -1, XC10BVC -2 | Lemon | Bella Vista, Corrientes | Argentina | A | + | + | + | |
| XC10BVC -3, XC10BVC -4, XC10BVC -5 | Orange | Bella Vista, Corrientes | Argentina | A | + | + | + | |
| XC10BVC -6, XC10BVC -7 | Grapefruit | Bella Vista, Corrientes | Argentina | A | + | + | + | |
| XC10BVC-8 | Tangerine | Bella Vista, Corrientes | Argentina | A | + | + | + | |
| XC6FT-1, XC6FT-2, XCT2, XCT3, XCT7, XCT9, XCT18, XCT22, XCT31, XCT33, XCT42 | Lemon Leave | Tucumán | Argentina | A | + | + | + | |
| XCT1, XCT17, XCT19, XCT21, XCT28, XCT29, | Lemon Fruit | Tucumán | Argentina | A | + | + | + | |
| XCT44 | Tangerine Leave | Tucumán | Argentina | A | + | + | + | |
| 306 (sequenced strain) | -- | -- | Brazil | A | + | + | + | |
| 625 | -- | Aratiba, Sao Paulo | Brazil | A | + | + | + | |
| 1637 | -- | Embaúba, Sao Paulo | Brazil | A | + | + | + | |
| 1740 | -- | -- | China | A | + | + | + | |
| 1801 | -- | -- | Oman | A* | + | + | + | |
| Xanthomonas fuscans subsp. Aurantifolii | B832 | -- | -- | Argentina | B | + | + | + |
| 382,1473 | -- | -- | Brazil | C | + | + | + | |
| Xanthomonas axonopodis pv. Citrumelo | 1925 | -- | -- | USA | -- | - | - | - |
For each isolate CBC-LAMP reaction was performed in triplicate. When available, detailed data about the place of origin and type of sample is included. Gel: gel electrophoresis. LFD: lateral flow dipstick. SG: SYBRGreen.+: Positive reaction.-: Negative reaction.