Table 1.
Genotyping results of An. culicifacies samples by PCR-based assays and allelic association between L1014 and V1010 mutations as revealed by DNA sequencing
| L1014 | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Genotyping methods |
Genotypes | ||||||||
| L/L | L/F | L/S | F/S | F/F | S/S | Total | |||
| PCR-based assays | 176 (0.786) | 26 (0.116) | 18 (0.080) | 2 (0.009) | 2 (0.009) | 0 (0.000) | 224 | ||
| V/V | 64 | 26 | 0 | 0 | 2 | 0 | 92 | ||
| V/L | 0 | 0 | 17* | 2 | 0 | 0 | 19* | ||
| DNA-sequencing | V1010 | ||||||||
| L/L | 0 | 0 | 0 | 0 | 0 | 0 | 0 | ||
| Total | 64 | 26 | 17 | 2 | 2 | 0 | 111 | ||
Figures in parenthesis indicate genotype frequencies. Letters L, F, S and V refer to leucine, phenylalanine, serine and valine amino acids, respectively.
*In two samples V1010L substitution was due to 3028G>C transversion and in rest samples due to 3028G>T transversion.