Figure 7. Quantitative transcript profiling of EDS1-dependent genes in EDS1-YFP-GR transgenic lines after triggering RPS4 resistance.
Four-week-old plants untreated (grey bars) or pre-treated with Dexamethasone (Dex) for 5 h (black bars) were inoculated with Pst DC3000 AvrRps4. Leaf samples were collected from untreated plants (T0) and 8 h after pathogen inoculation. Bars represent means and standard deviation of two or three biological replicates. Relative transcript levels were determined by qRT-PCR and normalized using the internal control UBIQUITIN. Expression was normalized in different samples using the endogenous control gene UBIQUITIN. (A) Up-regulated genes. (B) Down-regulated genes.