Figure 2. Testing for the activity of anti-Helix-3 antibodies: pAb RVC does not recognize PrPSc generated in prion infected brains.
(A) Activity of pAb RVC and RTC, as compared to the established mAb 6H4, against normal brain homogenates from bovine, mouse, humans and hamster. (B) Human and mouse normal brain homogenates were immunoblotted with pAb RVC and RTC alone or in the presence of diverse Helix-3 PrP peptides (see Figure 1C for the peptide sequences) (C) Brain homogenates from mice, hamster and humans (normal, prion-infected and prion-infected digested with proteinase K), were immunoblotted with mAb 6H4, with pAb RTC or RVC, or with rec Ab R1. D) Mouse scrapie (RML) and human CJD brains (E200K) were digested with PK, processed for MMA reduction as described in the methods and immunoblotted with α PrP mAb 6H4, IPC2, or pAb RVC.