Fig. 8.

Catalase blocks SS ion reduction in PKG-dependent VASP phosphorylation in VSMCs. VSMCS (p4) were treated with SS ions (0.1 ppm) for 6 h in the presence or absence of either 100 units/ml or 500 units/ml catalase. Dilute acid is a control for SS ion preparation. 8pCPT-cGMP (1 mmol/l) is a positive control for PKG-dependent VASP phosphorylation. Cell lysates of pooled triplicate samples were analyzed for phospho-VASP (ser239) by immunoblotting (n = 3). Blots were stripped and reprobed for β-tubulin.